CRISPR/Cas9 Practical for Beginners: Step by Step Hands on

Unlock the power of gene editing with CRISPR Practical for Beginners! This is a hands-on, step-by-step journey designed to take you from zero lab experience to performing CRISPR gene editing yourself. Forget dry theory – this course is all about doing.

What will you learn?

• Primer Designing: Master the essential first step in any gene editing project. Learn to create effective DNA primers for targeted gene manipulation.
• PCR (Polymerase Chain Reaction): Understand and execute PCR, the core technique for amplifying DNA – the heart of molecular biology.
• Gel Electrophoresis: Visualize your DNA! Learn to run gel electrophoresis to confirm your PCR products and assess their size.
• CRISPR/Cas9 Principles: Dive into the fascinating story of gene editing, exploring the science behind CRISPR/Cas9 technology.
• gRNA Designing: Craft your guide RNA (gRNA) – the key to directing the CRISPR/Cas9 system to your target DNA sequence.
• Microinjection: Learn the technique of microinjection to introduce CRISPR/Cas9 components into cells or zygotes.
• CRISPR/Cas9 Gene Editing: Perform real genome editing experiments and witness the power of precise DNA modification.
• Mutation Screening: Develop skills in mutation screening to validate your edits and confirm successful gene modification.

Why take this course?

Gain practical skills that are highly valuable and in-demand. CRISPR skills can significantly boost your career prospects. The confidence to say "I performed CRISPR myself" is a game-changer!

Who is this for?

This course is perfect for:

• Students eager to explore cutting-edge biology.
• Beginners with no prior lab or biology experience.
• Anyone with a curiosity about gene editing and a desire to learn by doing.

Requirements:

• No prior lab experience needed.
• No advanced biology background required – just a willingness to learn!

About the Instructor:

I'm Faheem Kanwal, a former student who overcame initial challenges to become a CRISPR expert. My journey took me from a small lab to prestigious institutions like Shanghai Jiao Tong University and Tsinghua University China, where I'm currently developing future cancer therapeutics using CRISPR. I'll share my step-by-step learning process – the mistakes and the breakthroughs – to guide you effectively.

My Promise:

By the end of this course, you won't just know about CRISPR. You'll perform CRISPR with your own hands.